gcIgM 小鼠单克隆抗体
产品名称： gcIgM 小鼠单克隆抗体
英文名称： gcIgM Mouse monoclonal antibody
产品编号： LPMA10232
产品价格： null
产品产地： 中国
品牌商标： Ablepscience
更新时间： null
使用范围：
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- 所在区域 : 湖北
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产品索引
Grass carp IgM
产品数据
货号 LPMA10232 文献 详细请查看官网 产品名称 Anti gcIgM mouse monoclonal antibody 中文名称 gcIgM 小鼠单克隆抗体 规格 100ug 适应种属 Grass carp 纯化方法 Protein G purified from mice ascites UniProt ID 浓度 1mg/ml 宿主 Mouse 克隆性 Monoclonal 亚型 IgG1 免疫原 GST-gcIgM 分子量 验证应用 Elisa,WB,flow,IHC 验证应用 Liquid 建议稀释比例 Elisa:1:1000~5000;Flow:1:100~400,IHC:1:50~100 存储条件 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 °C for frequent use. Store at -20 to -80 °C for twelve months from the date of receipt. 偶联物 Unconjugate 储存溶液 PBS, pH7.4, containing 0.05% proclin300, 50% glycerol. 运输条件 所有可选规格：常温运输或根据产品储存温度要求用蓝冰运输。 注意事项 该产品仅供科研使用
背景介绍
Grass carp IgM and identified two different IgM+ cell subsets:IgM+lymphocytes (Lym), resembling nave/mature B cells, and IgM+ myeloid cells (Mye),resembling plasma cells. Like plasma cells in mammals, the size of IgM+ Mye is significantly larger than that of IgM+ Lym, as revealed by flow cytometric analysisand transmission electron microscopy. The IgM+ Mye were further verified as plasma cells because they showed gene expression patterns similar with those of human plasma cells and a great capacity to secrete IgM. Like mammalian IgM+ and IgA+ plasma cells, not IgG+ plasma cells, grass carp IgM+ Mye also expressed membrane immunoglobulins, a feature conserved in IgM+ plasma cells in vertebrates. Furthermore, recombinant CD40L or IL-21 alone could induce the plasma cell generation and IgM secretion, while the combination of CD40L and IL-21 had greater effect on IgM secretion, but not on plasma cell generation. This study fills an important gap in the knowledge of plasma cells in teleost fish and provides criticalinsights into the conserved evolution of IgM+ plasma cells in vertebrates.