Anti-Active Rab5 Mouse Monoclonal Antibody
产品名称： Anti-Active Rab5 Mouse Monoclonal Antibody
英文名称： Anti-Active Rab5 Mouse Monoclonal Antibody
产品编号： 26911
产品价格： null
产品产地： 美国
品牌商标： NewEastbio
更新时间： null
使用范围： IP, IHC
武汉纽斯特生物技术有限公司
- 联系人 :
- 地址 : 武汉市东湖新技术开发区光谷生物城B3-3栋2-3楼
- 邮编 : 430074
- 所在区域 : 湖北
- 电话 : 186****7297 点击查看
- 传真 : 点击查看
- 邮箱 : neweast3@126.com
| Catalog Number: 26911 |
| Gene Symbol: RAB5 |
| Description: Anti-Active Rab5 Mouse Monoclonal Antibody |
| Background: Rab5 is a member of Rab-family GTPases. Rab5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway. |
| Immunogen: Recombinant full length protein of active Gao |
| Applications: IP, IHC |
| Recommended Dilutions: 1 µg for ~2 mg total cellular proteins for IP 1:100 dilution for IHC |
| Concentration: 1 mg/ml |
| Host: mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG1 |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti-active Rab5 antibody recognizes active Rab5 proteins of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Immunoprecipitation/Western blot: |
 |
Rab5 activation assay. Purified full-length Rab5A proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-active Rab5 monoclonal antibody (Cat. No. 26911). Immunoblot was with an anti-Rab5 polyclonal antibody (Cat. No. 21151). |
| Publications: |
| 1. VASP promotes TGF-β activation of hepatic stellate cells by regulating Rab11 dependent plasma membrane targeting of TGF-β receptors Hepatology Volume 61, Issue 1, pages 361–374, January 2015 |
| 2. Rab5 Activity Regulates GLUT4 Sorting into Insulin-Responsive and Non-Insulin-Responsive Endosomal Compartments: A Potential Mechanism for Development of Insulin Resistance Endocrinology. 2014 Sep;155(9):3315-28 |