Suggested Dilution Range:  1:500-1:5,000 for immunohisto/cytochemistry
1:5,000-1:100,000 for ELISA and Western blotting with chromogenic substrates
1:10,000-1:200,000 for Western blotting with ECL substrates

Peroxidase:  Punified from horseradish root

Buffer:  0.01M Sodium Phosphate,0.25M NaCl,Ph7.6

Stabilizer:  15mg/ml Bovine Serum Albumin(IgG-Free,Protease-Free)

Preservative:  None(Warning:use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.)
Reconstitution and Storage: Store freeze-dried powder at 2-8℃.When ready to use,rehydrate with 2.0ml d.water and centrifuge if not clear.Product is stable for about 6 weeks at2-8℃ as an undiluted liquid.Prepare working dilution fresh each day. For exiended storage after rehydration,add an equal volume of glycerol(ACS grade or better)for a final concentration of 50%,and store at-20℃ as a liquid. Note:after the addition of glycerol,the concentration of protein and buffer salts is one-half of the original.Alternatively,aliquot and freeze the product at -70℃ or below in the absence of glycerol.Avoid repeated freezing and thawing.
Expiration date:  one year from date of rehydration.
 
Purity:  The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Antibody Specificity:  Based on immunoelectrophoresis and/or ELISA,the antibody reacts with whole molecule rabbit IgG.It also reacts with the light chains of other rabbit immunoglobulins.No antibody was detected against non-immunoglobulin serum proteins.
The antibody may cross-react with immunoglobulins from other species.
 
Country of Origin:  USA

Note:  For in vitro research use only,not for diagnostic or therapeutic use.This product is not a medical device.