Alternative Names:
aging-associated gene 9 protein; G3P; G3PD; GAPD; GAPDH; glyceraldehyde 3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; MGC88685
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Applications:
WB: 1:3000~1:10000, IHC: 1:200 , IF/ICC: 1:200, |
Reactivity:
H, R, M, Mk, Dg, Ch, Hm, Rabbit,Pg |
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| Western blot analysis of Hela (1), Rat brain (2), Rabbit Muscle(3), Sheep Muscle(4), and Mouse brain (5) with GAPDH mouse mAb. |
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Purification:
Affinity-chromatography |
Specificity:
GAPDH Mouse Monoclonal antibody detects endogenous levels of total GAPDH protein. |
Immunogen:
Full-length GAPDH protein of human. |
Description:
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively abundant expressed in almost cell types at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some pathology factors, such as hypoxia and diabetes, increased or decreased GAPDH expression in certain cell types.
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Storage Condition and Buffer:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
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